MASCOT Search Results

Search metadata
User : Ricarda
E-mail : 20200226_HeLa_beforeF3
MS data file : HeLa_before_F3.mgf
Database : Human-UniProt 20160413 (70,236 sequences; 23,750,883 residues)
Timestamp : 26 Feb 2020 at 04:28:37 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Variable modifications : Carbamidomethyl (C), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm (# 13C = 1)
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-QUAD-TOF
Number of queries : 7,243

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.

There are 653 peptide matches above identity threshold and 934 matches above homology threshold for 7,243 queries. Histogram score range is (0, 125). On average, individual ions scores > 30 (beyond green shading) indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Modification statistics
Modification Site Above thr.
Carbamidomethyl C 92
Oxidation M 13
Legend
Dupes Expect Rank U 1 2 Peptide
0.037 Expand2 GAYSLSLR significant
9 Expand1 GFFLFVEGGR top ranking
6.4e-05 Expand1 GSSIFGLAPGK significant and top ranking
1.3e-06 Expand1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-07 Expand1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-05 Expand1 U GSSIFGLAPGK unique
Expand2 5.7e-05 Expand1 LNTLETEEWFFK peptide has two duplicates
0.18 Expand1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (Expand) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (Collapse) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Taxonomy

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Protein families 1–10 (out of 237)

Page: 1 2 3 4 5 6 … 24 Next 

Expand1

Accession Score Description
1 P06733 707 ENOA_HUMAN Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2

Expand2

Accession Score Description
1 P68371 505 TBB4B_HUMAN Tubulin beta-4B chain OS=Homo sapiens GN=TUBB4B PE=1 SV=1

Expand3

Accession Score Description
1 H3BQN4 487 H3BQN4_HUMAN Fructose-bisphosphate aldolase OS=Homo sapiens GN=ALDOA PE=1 SV=1

Expand4

Accession Score Description
Family member distances as a dendrogram 1 P07195 381 LDHB_HUMAN L-lactate dehydrogenase B chain OS=Homo sapiens GN=LDHB PE=1 SV=2
2 P00338 332 LDHA_HUMAN L-lactate dehydrogenase A chain OS=Homo sapiens GN=LDHA PE=1 SV=2

Expand5

Accession Score Description
Family member distances as a dendrogram 1 P05787 378 K2C8_HUMAN Keratin, type II cytoskeletal 8 OS=Homo sapiens GN=KRT8 PE=1 SV=7
3 P08729 193 K2C7_HUMAN Keratin, type II cytoskeletal 7 OS=Homo sapiens GN=KRT7 PE=1 SV=5
2 B0YJC4 345 B0YJC4_HUMAN Vimentin OS=Homo sapiens GN=VIM PE=1 SV=1

Expand6

Accession Score Description
1 P10809 368 CH60_HUMAN 60 kDa heat shock protein, mitochondrial OS=Homo sapiens GN=HSPD1 PE=1 SV=2

Expand7

Accession Score Description
1 Q5VTE0 318 EF1A3_HUMAN Putative elongation factor 1-alpha-like 3 OS=Homo sapiens GN=EEF1A1P5 PE=5 SV=1

Expand8

Accession Score Description
1 Q04695 284 K1C17_HUMAN Keratin, type I cytoskeletal 17 OS=Homo sapiens GN=KRT17 PE=1 SV=2

Expand9

Accession Score Description
1 P60709 277 ACTB_HUMAN Actin, cytoplasmic 1 OS=Homo sapiens GN=ACTB PE=1 SV=1

Expand10

Accession Score Description
1 P25705 273 ATPA_HUMAN ATP synthase subunit alpha, mitochondrial OS=Homo sapiens GN=ATP5A1 PE=1 SV=1

Page: 1 2 3 4 5 6 … 24 Next 

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