MASCOT Search ResultsMASCOT Search Results| User | : | Ricarda |
|---|---|---|
| : | Seagrass_PAII | |
| MS data file | : | Haberlea_PAII.mgf |
| Database | : | Tair10 20150119 (35,386 sequences; 14,482,855 residues) |
| Timestamp | : | 26 Feb 2020 at 03:05:33 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Variable modifications | : | Carbamidomethyl (C), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm (# 13C = 1) |
| Fragment mass tolerance | : | ± 0.5 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-QUAD-TOF |
| Number of queries | : | 12,473 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.
There are 578 peptide matches above identity threshold and 788 matches above homology threshold for 12,473 queries. Histogram score range is (0, 127). On average, individual ions scores > 27 (beyond green shading) indicate identity or extensive homology (p<0.05).
[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Modification | Site | Above thr. |
|---|---|---|
| Oxidation | M | 24 |
| Carbamidomethyl | C | 1 |
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-05 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-06 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-07 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-05 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-05 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATCG00490.1 | 1001 | | Symbols: RBCL | ribulose-bisphosphate carboxylases | chrC:54958-56397 FORWARD LENGTH=479 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT3G09440.1 | 971 | | Symbols: | Heat shock protein 70 (Hsp 70) family protein | chr3:2903434-2905632 REVERSE LENGTH=649 | |
| 5 | AT5G28540.1 | 265 | | Symbols: BIP1 | heat shock protein 70 (Hsp 70) family protein | chr5:10540665-10543274 REVERSE LENGTH=669 | |
| 4 | AT1G16030.1 | 373 | | Symbols: Hsp70b | heat shock protein 70B | chr1:5502386-5504326 REVERSE LENGTH=646 | |
| 2 | AT3G12580.1 | 908 | | Symbols: HSP70, ATHSP70 | heat shock protein 70 | chr3:3991487-3993689 REVERSE LENGTH=650 | |
| 3 | AT5G02500.1 | 515 | | Symbols: HSC70-1, HSP70-1, AT-HSC70-1, HSC70 | heat shock cognate protein 70-1 | chr5:554055-556334 REVERSE LENGTH=651 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATCG00270.1 | 589 | | Symbols: PSBD | photosystem II reaction center protein D | chrC:32711-33772 FORWARD LENGTH=353 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT5G08670.1 | 503 | | Symbols: | ATP synthase alpha/beta family protein | chr5:2818395-2821149 REVERSE LENGTH=556 | |
| 2 | ATCG00480.1 | 404 | | Symbols: ATPB, PB | ATP synthase subunit beta | chrC:52660-54156 REVERSE LENGTH=498 | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT3G12780.1 | 459 | | Symbols: PGK1 | phosphoglycerate kinase 1 | chr3:4061127-4063140 REVERSE LENGTH=481 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT5G13490.1 | 455 | | Symbols: AAC2 | ADP/ATP carrier 2 | chr5:4336034-4337379 FORWARD LENGTH=385 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATCG00280.1 | 450 | | Symbols: PSBC | photosystem II reaction center protein C | chrC:33720-35141 FORWARD LENGTH=473 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT2G21330.1 | 445 | | Symbols: FBA1 | fructose-bisphosphate aldolase 1 | chr2:9128416-9130152 REVERSE LENGTH=399 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT1G53240.1 | 425 | | Symbols: mMDH1 | Lactate/malate dehydrogenase family protein | chr1:19854966-19856802 REVERSE LENGTH=341 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT3G09640.1 | 403 | | Symbols: APX2, APX1B | ascorbate peroxidase 2 | chr3:2956301-2958163 FORWARD LENGTH=251 |
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