MASCOT Search ResultsMASCOT Search Results| User | : | Ricarda |
|---|---|---|
| : | Haberlea_fresh | |
| MS data file | : | Haberlea_fresh.mgf |
| Database | : | Tair10 20150119 (35,386 sequences; 14,482,855 residues) |
| Timestamp | : | 26 Feb 2020 at 02:56:46 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Variable modifications | : | Carbamidomethyl (C), Oxidation (M) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 20 ppm (# 13C = 1) |
| Fragment mass tolerance | : | ± 0.5 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-QUAD-TOF |
| Number of queries | : | 12,833 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.
There are 259 peptide matches above identity threshold and 401 matches above homology threshold for 12,833 queries. Histogram score range is (0, 145). On average, individual ions scores > 27 (beyond green shading) indicate identity or extensive homology (p<0.05).
[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Modification | Site | Above thr. |
|---|---|---|
| Oxidation | M | 19 |
| Carbamidomethyl | C | 3 |
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-05 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-06 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-07 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-05 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-05 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT3G23990.1 | 1144 | | Symbols: HSP60, HSP60-3B | heat shock protein 60 | chr3:8669013-8672278 FORWARD LENGTH=577 |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT5G08680.1 | 994 | | Symbols: | ATP synthase alpha/beta family protein | chr5:2821992-2824683 FORWARD LENGTH=559 | |
| 2 | ATCG00480.1 | 501 | | Symbols: ATPB, PB | ATP synthase subunit beta | chrC:52660-54156 REVERSE LENGTH=498 | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT2G13560.1 | 350 | | Symbols: NAD-ME1 | NAD-dependent malic enzyme 1 | chr2:5650089-5655103 FORWARD LENGTH=623 |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT5G65750.1 | 303 | | Symbols: | 2-oxoglutarate dehydrogenase, E1 component | chr5:26304212-26307947 FORWARD LENGTH=1025 |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATCG00680.1 | 286 | | Symbols: PSBB | photosystem II reaction center protein B | chrC:72371-73897 FORWARD LENGTH=508 |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT1G06430.1 | 272 | | Symbols: FTSH8 | FTSH protease 8 | chr1:1960214-1962525 REVERSE LENGTH=685 |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | ATCG00280.1 | 249 | | Symbols: PSBC | photosystem II reaction center protein C | chrC:33720-35141 FORWARD LENGTH=473 |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT2G18450.1 | 243 | | Symbols: SDH1-2 | succinate dehydrogenase 1-2 | chr2:7997510-8000801 REVERSE LENGTH=632 |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT4G35090.1 | 211 | | Symbols: CAT2 | catalase 2 | chr4:16700937-16703215 REVERSE LENGTH=492 |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | AT5G13490.1 | 210 | | Symbols: AAC2 | ADP/ATP carrier 2 | chr5:4336034-4337379 FORWARD LENGTH=385 |
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