MASCOT Search Results

Search metadata
User : Nathan
E-mail : Hela_20200219_afterF12_2
MS data file : HeLa_post_f12_2.mgf
Database : Human-UniProt 20160413 (70,236 sequences; 23,750,883 residues)
Timestamp : 24 Feb 2020 at 06:07:55 GMT
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Search parameters
Type of search : MS/MS Ion Search
Enzyme : Trypsin
Variable modifications : Carbamidomethyl (C), Oxidation (M)
Mass values : Monoisotopic
Protein mass : Unrestricted
Peptide mass tolerance : ± 20 ppm (# 13C = 1)
Fragment mass tolerance : ± 0.6 Da
Max missed cleavages : 1
Instrument type : ESI-QUAD-TOF
Number of queries : 9,975

Score distribution

Peptide score distribution

Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.

There are 3,415 peptide matches above identity threshold and 4,668 matches above homology threshold for 9,975 queries. Histogram score range is (0, 130). On average, individual ions scores > 30 (beyond green shading) indicate identity or extensive homology (p<0.05).

Protein score distribution for the first 50 proteins

[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.

Modification statistics
Modification Site Above thr.
Carbamidomethyl C 644
Oxidation M 16
Legend
Dupes Expect Rank U 1 2 Peptide
0.037 Expand2 GAYSLSLR significant
9 Expand1 GFFLFVEGGR top ranking
6.4e-05 Expand1 GSSIFGLAPGK significant and top ranking
1.3e-06 Expand1 X SSGTSYPDVLK peptide is found in all proteins in family member 1
6.2e-07 Expand1 / VCNYVSWIK peptide is found in some but not all proteins in family member 2
6.4e-05 Expand1 U GSSIFGLAPGK unique
Expand2 5.7e-05 Expand1 LNTLETEEWFFK peptide has two duplicates
0.18 Expand1 LNTLETEEWFFK duplicate peptide

Right-facing triangle (Expand) in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (Collapse) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.

Protein Family Summary

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Taxonomy

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Protein families 1–10 (out of 358)

Page: 1 2 3 4 5 6 … 36 Next 

Expand1

Accession Score Description
1 P06733 3944 ENOA_HUMAN Alpha-enolase OS=Homo sapiens GN=ENO1 PE=1 SV=2

Expand2

Accession Score Description
Family member distances as a dendrogram 1 P60709 3325 ACTB_HUMAN Actin, cytoplasmic 1 OS=Homo sapiens GN=ACTB PE=1 SV=1
2 P62736 1190 ACTA_HUMAN Actin, aortic smooth muscle OS=Homo sapiens GN=ACTA2 PE=1 SV=1

Expand3

Accession Score Description
Family member distances as a dendrogram 1 P14618 2925 KPYM_HUMAN Pyruvate kinase PKM OS=Homo sapiens GN=PKM PE=1 SV=4
2 P30613 50 KPYR_HUMAN Pyruvate kinase PKLR OS=Homo sapiens GN=PKLR PE=1 SV=2

Expand4

Accession Score Description
Family member distances as a dendrogram 1 P07437 2830 TBB5_HUMAN Tubulin beta chain OS=Homo sapiens GN=TUBB PE=1 SV=2
2 P68371 2776 TBB4B_HUMAN Tubulin beta-4B chain OS=Homo sapiens GN=TUBB4B PE=1 SV=1
3 P04350 2263 TBB4A_HUMAN Tubulin beta-4A chain OS=Homo sapiens GN=TUBB4A PE=1 SV=2

Expand5

Accession Score Description
1 P04406 2754 G3P_HUMAN Glyceraldehyde-3-phosphate dehydrogenase OS=Homo sapiens GN=GAPDH PE=1 SV=3

Expand6

Accession Score Description
Family member distances as a dendrogram 1 F5H5D3 2083 F5H5D3_HUMAN Tubulin alpha-1C chain OS=Homo sapiens GN=TUBA1C PE=1 SV=1
2 P68366 1625 TBA4A_HUMAN Tubulin alpha-4A chain OS=Homo sapiens GN=TUBA4A PE=1 SV=1

Expand7

Accession Score Description
Family member distances as a dendrogram 1 P05787 2082 K2C8_HUMAN Keratin, type II cytoskeletal 8 OS=Homo sapiens GN=KRT8 PE=1 SV=7
3 P08670 1136 VIME_HUMAN Vimentin OS=Homo sapiens GN=VIM PE=1 SV=4
2 P08729 1437 K2C7_HUMAN Keratin, type II cytoskeletal 7 OS=Homo sapiens GN=KRT7 PE=1 SV=5

Expand8

Accession Score Description
Family member distances as a dendrogram 1 P08238 2077 HS90B_HUMAN Heat shock protein HSP 90-beta OS=Homo sapiens GN=HSP90AB1 PE=1 SV=4
3 P14625 553 ENPL_HUMAN Endoplasmin OS=Homo sapiens GN=HSP90B1 PE=1 SV=1
2 P07900 1796 HS90A_HUMAN Heat shock protein HSP 90-alpha OS=Homo sapiens GN=HSP90AA1 PE=1 SV=5

Expand9

Accession Score Description
1 P10809 1884 CH60_HUMAN 60 kDa heat shock protein, mitochondrial OS=Homo sapiens GN=HSPD1 PE=1 SV=2

Expand10

Accession Score Description
1 P00558 1880 PGK1_HUMAN Phosphoglycerate kinase 1 OS=Homo sapiens GN=PGK1 PE=1 SV=3

Page: 1 2 3 4 5 6 … 36 Next 

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