MASCOT Search ResultsMASCOT Search Results| User | : | Lawrence |
|---|---|---|
| : | 21730852@student.uwa.edu,au | |
| Search title | : | young_flag_leaf_12-1 |
| MS data file | : | YOUNG_FLAG_LEAF_12.mgf |
| Database | : | IWGSC_RefSeq_1.0_Release40 20180925 (137,029 sequences; 60,328,507 residues) |
| Timestamp | : | 24 Feb 2020 at 04:19:10 GMT |
| Warning | : | Peptide mass of 16679 is too big. The maximum mass allowed is 15998. (Or incorrect data format) |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Variable modifications | : | Acetyl (K), Acetyl (N-term), Oxidation (HW), Oxidation (M), Phospho (ST), Phospho (Y) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 100 ppm (# 13C = 1) |
| Fragment mass tolerance | : | ± 0.5 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-QUAD-TOF |
| Number of queries | : | 11,724 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.
There are 780 peptide matches above identity threshold and 2,670 matches above homology threshold for 11,724 queries. Histogram score range is (0, 136). On average, individual ions scores > 49 (beyond green shading) indicate identity or extensive homology (p<0.05).
[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Modification | Site | Above thr. |
|---|---|---|
| Oxidation | M | 230 |
| Acetyl | N-term | 61 |
| Acetyl | K | 24 |
| Phospho | S | 17 |
| Oxidation | W | 15 |
| Phospho | T | 13 |
| Oxidation | H | 10 |
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-05 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-06 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-07 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-05 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-05 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
) indicates the content is expanded and can be collapsed. For more details about particular columns, see results format help.
1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS3B01G186100.1 | 2963 | pep chromosome:IWGSC:3B:201498059:201500035:1 gene:TraesCS3B01G186100 transcript:TraesCS3B01G186100.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 4 | TraesCS6A01G291700.1 | 49 | pep chromosome:IWGSC:6A:523643588:523643910:-1 gene:TraesCS6A01G291700 transcript:TraesCS6A01G291700.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS1D01G169900.1 | 252 | pep chromosome:IWGSC:1D:244392437:244393366:1 gene:TraesCS1D01G169900 transcript:TraesCS1D01G169900.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS5D01G425100.1 | 1624 | pep chromosome:IWGSC:5D:483801282:483802712:-1 gene:TraesCS5D01G425100 transcript:TraesCS5D01G425100.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2D01G065200.1 | 1050 | pep chromosome:IWGSC:2D:27859670:27860982:-1 gene:TraesCS2D01G065200 transcript:TraesCS2D01G065200.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS2A01G067000.1 | 1045 | pep chromosome:IWGSC:2A:30079492:30080632:-1 gene:TraesCS2A01G067000 transcript:TraesCS2A01G067000.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS5A01G165400.2 | 1048 | pep chromosome:IWGSC:5A:353988495:353989233:1 gene:TraesCS5A01G165400 transcript:TraesCS5A01G165400.2 gene_biotype:protein_coding transcript_biotype:protein_coding | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2A01G247300.3 | 787 | pep chromosome:IWGSC:2A:365284650:365285722:-1 gene:TraesCS2A01G247300 transcript:TraesCS2A01G247300.3 gene_biotype:protein_coding transcript_biotype:protein_coding |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2B01G342200.1 | 741 | pep chromosome:IWGSC:2B:488191847:488193329:1 gene:TraesCS2B01G342200 transcript:TraesCS2B01G342200.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS2A01G344600.1 | 652 | pep chromosome:IWGSC:2A:581941117:581942597:1 gene:TraesCS2A01G344600 transcript:TraesCS2A01G344600.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS4B01G323000.1 | 424 | pep chromosome:IWGSC:4B:613374537:613376620:1 gene:TraesCS4B01G323000 transcript:TraesCS4B01G323000.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS4D01G107400.1 | 571 | pep chromosome:IWGSC:4D:85590936:85593478:1 gene:TraesCS4D01G107400 transcript:TraesCS4D01G107400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS3B01G025200.1 | 54 | pep chromosome:IWGSC:3B:10953636:10956618:1 gene:TraesCS3B01G025200 transcript:TraesCS3B01G025200.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS5B01G115300.1 | 257 | pep chromosome:IWGSC:5B:192348939:192350202:-1 gene:TraesCS5B01G115300 transcript:TraesCS5B01G115300.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2A01G342600.1 | 568 | pep chromosome:IWGSC:2A:579616065:579616954:-1 gene:TraesCS2A01G342600 transcript:TraesCS2A01G342600.1 gene_biotype:protein_coding transcript_biotype:protein_coding |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2D01G229600.1 | 563 | pep chromosome:IWGSC:2D:199356342:199357421:1 gene:TraesCS2D01G229600 transcript:TraesCS2D01G229600.1 gene_biotype:protein_coding transcript_biotype:protein_coding |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS4A01G093100.1 | 501 | pep chromosome:IWGSC:4A:100634474:100636136:-1 gene:TraesCS4A01G093100 transcript:TraesCS4A01G093100.1 gene_biotype:protein_coding transcript_biotype:protein_coding |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCSU01G063400.1 | 463 | pep chromosome:IWGSC:Un:49995598:49998392:-1 gene:TraesCSU01G063400 transcript:TraesCSU01G063400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS7A01G071100.2 | 206 | pep chromosome:IWGSC:7A:36859842:36862632:-1 gene:TraesCS7A01G071100 transcript:TraesCS7A01G071100.2 gene_biotype:protein_coding transcript_biotype:protein_coding | |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2A01G254600.3 | 443 | pep chromosome:IWGSC:2A:387775955:387776908:1 gene:TraesCS2A01G254600 transcript:TraesCS2A01G254600.3 gene_biotype:protein_coding transcript_biotype:protein_coding |
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