MASCOT Search ResultsMASCOT Search Results| User | : | Lawrence |
|---|---|---|
| : | 21730852@student.uwa.edu,au | |
| Search title | : | young_flag_leaf_11-1 |
| MS data file | : | YOUNG_FLAG_LEAF_11.mgf |
| Database | : | IWGSC_RefSeq_1.0_Release40 20180925 (137,029 sequences; 60,328,507 residues) |
| Timestamp | : | 24 Feb 2020 at 04:37:04 GMT |
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| Type of search | : | MS/MS Ion Search |
|---|---|---|
| Enzyme | : | Trypsin |
| Variable modifications | : | Acetyl (K), Acetyl (N-term), Oxidation (HW), Oxidation (M), Phospho (ST), Phospho (Y) |
| Mass values | : | Monoisotopic |
| Protein mass | : | Unrestricted |
| Peptide mass tolerance | : | ± 100 ppm (# 13C = 1) |
| Fragment mass tolerance | : | ± 0.5 Da |
| Max missed cleavages | : | 1 |
| Instrument type | : | ESI-QUAD-TOF |
| Number of queries | : | 11,997 |
Peptide score distribution. Ions score is −10log(P), where P is the probability that the observed match is a random event.
There are 683 peptide matches above identity threshold and 2,559 matches above homology threshold for 11,997 queries. Histogram score range is (0, 112). On average, individual ions scores > 48 (beyond green shading) indicate identity or extensive homology (p<0.05).
[Deprecated] Protein score distribution. Score distribution for family members in the first 50 proteins. Protein scores are derived from ions scores as a non-probabilistic basis for ranking protein families.
| Modification | Site | Above thr. |
|---|---|---|
| Oxidation | M | 209 |
| Acetyl | N-term | 74 |
| Oxidation | W | 31 |
| Phospho | S | 22 |
| Acetyl | K | 19 |
| Phospho | T | 18 |
| Oxidation | H | 14 |
| Phospho | Y | 4 |
| Dupes | Expect | Rank | U | 1 | 2 | Peptide | |
|---|---|---|---|---|---|---|---|
| 0.037 |  2 |
GAYSLSLR | significant | ||||
| 9 | 1 |
GFFLFVEGGR | top ranking | ||||
| 6.4e-05 | 1 |
GSSIFGLAPGK | significant and top ranking | ||||
| 1.3e-06 | 1 |
 |
SSGTSYPDVLK | peptide is found in all proteins in family member 1 | |||
| 6.2e-07 | 1 |
 |
VCNYVSWIK | peptide is found in some but not all proteins in family member 2 | |||
| 6.4e-05 | 1 |
U | GSSIFGLAPGK | unique | |||
| 2 |
5.7e-05 | 1 |
LNTLETEEWFFK | peptide has two duplicates | |||
| 0.18 | 1 |
LNTLETEEWFFK | duplicate peptide |
Right-facing triangle () in the Dupes or Rank column indicates content that can be expanded by clicking on it. Down-facing triangle (
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1| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2D01G284400.1 | 1352 | pep chromosome:IWGSC:2D:359630619:359632115:1 gene:TraesCS2D01G284400 transcript:TraesCS2D01G284400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS1D01G171700.1 | 89 | pep chromosome:IWGSC:1D:244951405:244952100:-1 gene:TraesCS1D01G171700 transcript:TraesCS1D01G171700.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS1A01G379000.1 | 203 | pep chromosome:IWGSC:1A:551320471:551323724:-1 gene:TraesCS1A01G379000 transcript:TraesCS1A01G379000.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
2| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS5D01G425100.1 | 1151 | pep chromosome:IWGSC:5D:483801282:483802712:-1 gene:TraesCS5D01G425100 transcript:TraesCS5D01G425100.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS3B01G186100.1 | 536 | pep chromosome:IWGSC:3B:201498059:201500035:1 gene:TraesCS3B01G186100 transcript:TraesCS3B01G186100.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 3 | TraesCS5D01G010200.1 | 418 | pep chromosome:IWGSC:5D:6217773:6219137:-1 gene:TraesCS5D01G010200 transcript:TraesCS5D01G010200.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
3| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS4D01G135000.3 | 670 | pep chromosome:IWGSC:4D:121816441:121818125:1 gene:TraesCS4D01G135000 transcript:TraesCS4D01G135000.3 gene_biotype:protein_coding transcript_biotype:protein_coding |
4| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS5A01G424400.1 | 664 | pep chromosome:IWGSC:5A:609867425:609872445:1 gene:TraesCS5A01G424400 transcript:TraesCS5A01G424400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS2A01G250800.1 | 175 | pep chromosome:IWGSC:2A:378850107:378853251:-1 gene:TraesCS2A01G250800 transcript:TraesCS2A01G250800.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
5| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS5D01G458900.1 | 613 | pep chromosome:IWGSC:5D:503315316:503316830:1 gene:TraesCS5D01G458900 transcript:TraesCS5D01G458900.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS5D01G459400.1 | 442 | pep chromosome:IWGSC:5D:503341255:503342808:1 gene:TraesCS5D01G459400 transcript:TraesCS5D01G459400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
6| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS3D01G053400.1 | 560 | pep chromosome:IWGSC:3D:21903938:21905358:1 gene:TraesCS3D01G053400 transcript:TraesCS3D01G053400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS1D01G182600.1 | 443 | pep chromosome:IWGSC:1D:254497201:254498293:1 gene:TraesCS1D01G182600 transcript:TraesCS1D01G182600.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
7| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS4D01G319400.1 | 507 | pep chromosome:IWGSC:4D:483137131:483138993:1 gene:TraesCS4D01G319400 transcript:TraesCS4D01G319400.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
| 2 | TraesCS2B01G342200.1 | 351 | pep chromosome:IWGSC:2B:488191847:488193329:1 gene:TraesCS2B01G342200 transcript:TraesCS2B01G342200.1 gene_biotype:protein_coding transcript_biotype:protein_coding | |
8| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS2A01G247300.3 | 494 | pep chromosome:IWGSC:2A:365284650:365285722:-1 gene:TraesCS2A01G247300 transcript:TraesCS2A01G247300.3 gene_biotype:protein_coding transcript_biotype:protein_coding |
9| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS6A01G209800.1 | 479 | pep chromosome:IWGSC:6A:380110280:380111692:1 gene:TraesCS6A01G209800 transcript:TraesCS6A01G209800.1 gene_biotype:protein_coding transcript_biotype:protein_coding |
10| Accession | Score | Description | ||
|---|---|---|---|---|
| 1 | TraesCS7D01G066500.1 | 430 | pep chromosome:IWGSC:7D:37285606:37288702:-1 gene:TraesCS7D01G066500 transcript:TraesCS7D01G066500.1 gene_biotype:protein_coding transcript_biotype:protein_coding |
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